Part:BBa_K274004:Experience
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how you used this part and how it worked out.
Applications of BBa_K274004
User Reviews
UNIQ4f8cf33e271d073c-partinfo-00000000-QINU UNIQ4f8cf33e271d073c-partinfo-00000001-QINU
E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
Karina Arnesen, undergraduate, Alberta iGEM 2010
When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible.
Characterisation by 2011 iGEM NCTU_FORMOSA
This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.
We mutated the sequence to correct the Xba I restriction site .
And the sequence is made of vioABDE not vioABCE