Coding

Part:BBa_K633002

Designed by: Ricardo Camilo Chavez Martinez   Group: iGEM11_Tec-Monterrey   (2011-09-27)
Revision as of 03:56, 28 September 2011 by Registry (Talk | contribs)

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RBS+signal peptide phoA+Cellulase

We used Rybosome Binding Site BBa_B0034 designed by by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr, evaluated by Warsaw's 2010 team. we added membrane protein signal peptide phoA and cellulase immediately next to it

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1908
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 585
    Illegal AgeI site found at 228
    Illegal AgeI site found at 644
    Illegal AgeI site found at 1473
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation/cellulose
Parameters
None