Part:BBa_K678002

eGFP-PTS1 for mammalian cells

Vector expressing eGFP targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1) from the cytomegalovirus (CMV) promoter with the BGH polyA tail and with a hygromycin resistance cassette.

Usage and Biology

We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (Figure 1).

DTU-Denmark-2 2011 Figure 1: eGFP targeted to the peroxisomes of the mammalian cell line U-2 OS.

DTU-Denmark-2 2011 Figure 2: he mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2233
  Illegal SpeI site found at 49
  Illegal PstI site found at 2323
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2233
  Illegal SpeI site found at 49
  Illegal PstI site found at 2323
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2233
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2233
  Illegal SpeI site found at 49
  Illegal PstI site found at 2323
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2233
  Illegal SpeI site found at 49
  Illegal PstI site found at 2323
  Illegal NgoMIV site found at 3088
  
• 1000
  COMPATIBLE WITH RFC[1000]