Coding

Part:BBa_K530002

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-08-01)
Revision as of 02:00, 27 September 2011 by Danielw9 (Talk | contribs) (→‎Plasmid Map)

CRTI

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1108
    Illegal SapI site found at 508


Plasmid Map

CrtI Plasmid Map.png


Sequencing

CrtI Sequencing.png


This is the sequencing for colony 2. Colony 2 had a perfect match with the genomic DNA promoter.

[edit]
Categories
Parameters
None