Part:BBa_K516220:Experience

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Applications of BBa_K516220

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UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

Characterized with:

BBa_K516220 (wiki name: E24 ) p_Tet-RBS30-AiiA-TT
BBa_K516221 (wiki name: E25 ) p_Tet-RBS31-AiiA-TT
BBa_K516222 (wiki name: E26 ) p_Tet-RBS32-AiiA-TT
BBa_K516224 (wiki name: E27 ) p_Tet-RBS34-AiiA-TT

The AiiA enzyme activity has been characterized under the regulation of p_tet promoter, assaying its enzymatic activity.
The four measurement parts p_Tet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor.
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0.
A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2.
In this case, a significant difference in degradation between p_Tet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.

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