LasR autoinducing loop family

LasR autoinducing loop family

A LasR autoinducing loop consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this needs to be added to the medium in a concentration of 100nM.

Leakage of the LasR autoinducing loops

To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying an pSB1K3 plasmid with the autoinducing loop biobrick (different variants) and the plasmid pSB1A3 with an input(PLacI controlling LasR) as well as the output (PlasB controlling RFP-LVA). The measurements were done in a presence of 100nM PAI.

	no-tag	DAS	LVA
RBS 0,07	BBa_K607031	BBa_K607027	BBa_K607023
RBS 0,3	BBa_K607032	BBa_K607028	BBa_K607024
RBS 0,6	BBa_K607033	BBa_K607029	BBa_K607025
RBS 1	BBa_K607034	BBa_K607030	BBa_K607026
Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.