Coding

Part:BBa_K592002:Design

Designed by: Erik Lundin   Group: iGEM11_Uppsala-Sweden   (2011-09-09)
Revision as of 09:17, 21 September 2011 by SunLei (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K592002 short</partinfo> <partinfo>BBa_K592002 SequenceAndFeatures</partinfo> ===Design Notes=== The source DNA (pJT122) contained illegal restriction site (EcoR...)

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ccaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source DNA (pJT122) contained illegal restriction site (EcoRI) that had to be removed. Point mutagenesis of A4C and A6C were performed.


Source

Synechocystic sp. PCC6803, Jeffrey Tabor's pJT122 plasmid

References

Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.