Coding
Part:BBa_K620000:Design
Designed by: Caltech iGEM 2011 Group: iGEM11_Caltech (2011-09-20)
DDT Dehydrochlorinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.
Source
This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.