Part:BBa_K523001

RBS + malS (E. coli periplasmic α-amylase)

E. coli periplasmic α-amylase gene malS (see [http://www.ncbi.nlm.nih.gov/nuccore/48994873?from=3735520&to=3737550&report=gbwithparts info from GenBank: U00096.2]).

The native ribosome binding site is present.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

While an amylase ought to be capable of degrading starch, the product protein is believed to be periplasmic and thus ought to only degrade starch if it leaks from the periplasm in significant quantities. Its natural function in E. coli presumably involves degrading shorter glucose chains: [http://dx.doi.org/10.1128/JB.00767-08 Lengsfeld et al (2008)] state that "MalS produces preferentially maltohexaose from longer maltodextrins in the periplasm".

The SignalP program predicts that a 17 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.

Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part BBa_K523006.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1233
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 369
  Illegal AgeI site found at 1534
  
• 1000
  COMPATIBLE WITH RFC[1000]