Reporter

Part:BBa_K592015

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 14:57, 18 September 2011 by SunLei (Talk | contribs)

amilCP Blue Coding Device

This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized.

The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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