Coding

Part:BBa_K584021

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-17)
Revision as of 14:16, 18 September 2011 by Vinchhi (Talk | contribs)

Ice Nucleating Protein Gene - inaZ

This part contains the Ice nuceating protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene repsonsible for producing the ice nucleation protein.

We would like to thank Prof. Dr. Gregory Gloor (http://www.academicbiography.uwo.ca/profile.php?&n=ggloor), for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

An article in which he uses the pUC1813ICE with inaZ can be found at http://www.ncbi.nlm.nih.gov/pubmed/10933828.

References

[1] Bassi AS Et al., Expression of single chain antibodies (ScFvs) for c-myc oncoprotein in recombinant Escherichia coli membranes by using the ice-nucleation protein of Pseudomonas syringae. Biotechnol Prog. 2000 Jul-Aug;16(4):557-63.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 646
    Illegal NgoMIV site found at 2158
    Illegal NgoMIV site found at 2302
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 2878
    Illegal AgeI site found at 417
    Illegal AgeI site found at 1822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 3338
    Illegal SapI.rc site found at 238


[edit]
Categories
Parameters
None