Composite

Part:BBa_K592014:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 13:12, 18 September 2011 by SunLei (Talk | contribs) (→‎Design Notes)

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HO1-pcyA, low expression, B1001 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the toxic nature of HO1 and pcyA enzymatic activities, we added a very weak promoter (J23113) to the PCB chromophore module. This part was designed to be inserted into the bacterial chromosome. Therefore, B1001 was used instead of B0015.

Source

Assembled using parts from the 2011 iGEM distribution.

References