Coding
FixJ

Part:BBa_K592005:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-17)
Revision as of 14:25, 17 September 2011 by SunLei (Talk | contribs) (→‎References)

FixJ, the response regulator to YF1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 372
    Illegal NgoMIV site found at 466
    Illegal NgoMIV site found at 598
    Illegal AgeI site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 136


Design Notes

The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.


Source

FixJ comes from the genome of Bradyrhizobium japonicum.

References

Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.