Coding
YF1
Part:BBa_K592004:Design
Designed by: Lei Sun Group: iGEM11_Uppsala-Sweden (2011-09-17)
YF1 blue-light sensor protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
Design Notes
The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
Source
Ytva comes from the genome of Bacillus subtilis.
FixL derives from the genome of Bradyrhizobium japonicum.