Signalling

Part:BBa_K546000

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 19:20, 14 September 2011 by PimK (Talk | contribs)

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.

The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


[edit]
Categories
//classic/signalling/receiver
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
control(1) R0063 & (2) R0062
device_typefeedback
directionForward
latencySeconds
protein(1) LuxR & (2) luxI-LVA
signalling_molecule3OC6HSL (AHL)
switch_point(1) 2nM