Coding

Part:BBa_K523004

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-07-29)
Revision as of 10:19, 29 July 2011 by Registry (Talk | contribs)

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bglX for BioSandwich

This is a version of BBa_K523002 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.

There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.

The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1847
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None