Device
Part:BBa_K392045
Designed by: Tadashi Nakamura, Shuhei Yasumoto, Saya Kakuda, Takahiro Saka Group: iGEM10_Osaka (2010-11-07)
endoglucanase secretion testing construct for yeast
Yeast expression device containing C. fermi endoglucanase CenA with engineered secretion tag attached upstream to enable secretion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1604
Illegal NotI site found at 2748 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2661
Illegal BamHI site found at 1797
Illegal XhoI site found at 2159
Illegal XhoI site found at 2408 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
Illegal NgoMIV site found at 1939
Illegal NgoMIV site found at 2864 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 830
Illegal BsaI site found at 1843
[edit]
Categories
Parameters
None |