Regulatory

Part:BBa_K310000:Design

Designed by: Francis Lee   Group: iGEM10_UIUC-Illinois   (2010-09-15)
Revision as of 12:24, 6 November 2010 by Ifrancium (Talk | contribs) (→‎Design Notes)

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micA (sRNA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick prefix is attached just upstream of the sRNA. This leads to the addition of 8 nucleotides (scar coding) to the 5' end of the sRNA, TACTAGAG, when combined with an upstream part (ie-promoter). All sRNA's in E. coli carry an intrinsic terminator. The suffix is located ~20 bp downstream of this terminator.

An EcoRI site has been changed to a Gatttc site. K310000 is now RFC10 compatible.

Source

Escherichia coli MG1655

References