Regulatory

Part:BBa_K310017:Design

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-10-27)
Revision as of 12:12, 6 November 2010 by Ifrancium (Talk | contribs) (→‎References)

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gadX 3'UTR (gadY target)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gadX is known to have 3 different 3'UTR. The gadX Biobrick allows for all 3. For more information, see [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522195/?tool=pmcentrez]

Also, this part will add 8 nucleotides to 3'UTR as an artifact of the RFC10 Biobrick assembly (Scar coding)

Source

e. coli K-12 mg155

References

Opdyke JA, Kang JG, Storz G (2004). "GadY, a small-RNA regulator of acid response genes in Escherichia coli". J. Bacteriol. 186 (20): 6698–705. doi:10.1128/JB.186.20.6698-6705.2004. PMID 15466020.