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  • Part:BBa K4339008
    ...domain from the same protein. Each repetitive region begins with a glycine rich region and ends with between seven and nine alanines. ...between poly-alanine regions and hydrophilic interactions between glycine-rich regions confer the strength and elasticity of dragline silk respectively [3
    6 KB (862 words) - 13:22, 12 October 2022
  • Part:BBa K4417017
    ...rphology.Our engineered bacteria produce crystal-like colonies (on calcium rich LB plates) which points to increased CaCO3 precipitation caused by our gene ...e:Ca plate.jpg|600px|thumb|center|'''Figure 7:''' Precipitation on calcium rich agar plates by TU1.]]
    8 KB (1,186 words) - 14:45, 12 October 2022
  • Part:BBa K4417023
    ...phology. Our engineered bacteria produce crystal-like colonies (on calcium rich LB plates) which points to increased CaCO3 precipitation caused by our gene ...:Heiheihei.png|600px|thumb|center|'''Figure 6:''' Precipitation on calcium rich agar plates by CA17.]]
    5 KB (842 words) - 02:54, 14 October 2022
  • Part:BBa J23117
    ...and act as a programmable transcription regulator . Another promoter, PAM rich URS J23117Alt ( BBa_K1723005 ) was created by mutating sequences of the imp
    6 KB (973 words) - 11:22, 27 October 2020
  • Part:BBa K257001
    ...rminal domains and one C-terminal domain, separated by two flexible glycin-rich linkers. All three domains are indispensable for phage infectivity. *N1, N2 and N3 domain are linked by <b>flexible glycin-rich domains (G1 and G2)</b>. G1 is composed of four tandem copies of the sequen
    3 KB (428 words) - 17:17, 20 October 2009
  • Part:BBa K401001
    ...35.jpg|thumb|center|400px|'''Figure 2'''. The prion domain of Sup35 is Q/N Rich and has the ability to propogate the corresponding prion in the absence of ...e prionic system-containing yeasts could grow on the selective medium. The rich medium impose no restriction for the growth of the three strains.]]
    13 KB (1,991 words) - 17:47, 26 October 2017
  • Part:BBa K533006:Design
    ...he N-terminal to facilitate purification of the expressed protein. Proline rich sequence put here is much longer than the length required for SH3 binding, ...equence PVPPPVPPRRRP is from the structure of SH3 complexed with a proline rich peptide solved by Wittekind, ''et al''. The Kd value is determined by them
    1 KB (154 words) - 16:08, 5 October 2011
  • Part:BBa K1151001:Design
    ...Gilbert et al, Protein Hpn: cloning and characterization of a Histidine-Rich Metal-Binding Polypeptide in Helicobacter pylori and Helicobacter mustel 2. Ruiguang et al, Expression and characterization of a histidine-rich protein, Hpn: potential for Ni2+ storage in Helicobacter pylori. Biochem J.
    793 B (96 words) - 19:17, 2 October 2013
  • Part:BBa K1620000
    ...ion occurs at high levels in the first three hours, like element puspA, in rich medium. Indeed, seems that the crude amounts of GFP are higher in the puspA ...media. A significant difference was observed between pJ23101 and puspA in rich media, evidenced through t-test at 0.05% of significance. Statistical infer
    19 KB (2,830 words) - 19:48, 5 September 2015
  • Part:BBa K1723008:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:<br/>< ...ee that in presence of the complex BBa_K1723008-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more info
    2 KB (294 words) - 20:34, 18 September 2015
  • Part:BBa K1723002:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/ ...ee that in presence of the complex BBa_K1723002-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more info
    2 KB (313 words) - 20:32, 18 September 2015
  • Part:BBa K1723003:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/ ...ee that in presence of the complex BBa_K1723003-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informat
    2 KB (310 words) - 20:32, 18 September 2015
  • Part:BBa K1723004:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/ ...ee that in presence of the complex BBa_K1723004-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more info
    2 KB (313 words) - 20:33, 18 September 2015
  • Part:BBa K1723006:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:<br/>< ...ee that in presence of the complex BBa_K1723006-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more info
    2 KB (294 words) - 20:33, 18 September 2015
  • Part:BBa K1723007:Experience
    <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:<br/>< ...ee that in presence of the complex BBa_K1723007-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informat
    2 KB (293 words) - 20:33, 18 September 2015
  • Part:BBa K2014011
    ...7</b>, and <b>BBa_K2014012</b> in <i>E. coli </i> DH5α cells grown in two rich media, <b>LB and SB-PKB and in M9 minimal medium </b>upon induction with xy ...ferent variants of sfGFP ORFs during 6h culture of <i>E. coli</i> DH5α in rich media upon induction with D-xylose (0h) (0,4% final concentration). </font>
    4 KB (707 words) - 00:08, 22 October 2016
  • Part:BBa K2014012
    ...07</b>, and <b>BBa_K2014012</b> in <i>E. coli</i> DH5α cells grown in two rich media, <b>LB and SB-PKB and in M9 minimal medium </b>upon induction with xy ...ferent variants of sfGFP ORFs during 6h culture of <i>E. coli</i> DH5α in rich media upon induction with D-xylose (0h) (0,4% final concentration). </font>
    5 KB (760 words) - 00:19, 22 October 2016
  • Part:BBa K2282009
    ...uence is indeed located from -47 to -38 from the 5'UTR beginning and is AT rich. Sequences of the cold-induced elements are very close to the parts of the ...et al, Deletion analysis of cspA of Escherichia coli requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding regi
    2 KB (311 words) - 12:46, 25 October 2017
  • Part:BBa K2314831
    ...and can aid in RNAP stabilization to enhanced transcription rates. The AT-rich spacer containing 30 nucleotides has a better performance.[1]
    3 KB (457 words) - 19:22, 1 November 2017
  • Part:BBa M50497:Experience
    ...iplicates of each culture were compiled in a 96-well plate and grown in EZ Rich Media in a 37℃ shaking incubator for two days. A pColi-harboring culture ...duction. The same cultures used for the initial GFP assay were grown in EZ Rich Media with 12 mL 20% glucose and ampicillin for 16 hours and then diluted t
    5 KB (824 words) - 06:17, 11 December 2018

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