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- ...r. Urea-PAGE 15% of 19:1 (Acrylamide:Bisacrylamide) ratio. Electrophoresis ran for ~3 hours at 120 V.]] </li> Wang, X., Xu, X., Ma, Z., Huo, Y., Xiao, Z., Li, Y. and Wang, Y., 2020. Dynamic Mechanisms For Pre-Mirna Binding And Export By Exportin-21 KB (3,235 words) - 03:18, 28 October 2020
- ...the proteins were affinity-purified using Ni-NTA or Co-NTA beads. Then we ran the SDS-PAGE gel to Coomassie brilliant blue staining and Western Blotting. [2] Wang, P., Tang, W., Li, Z., Zou, Z., Zhou, Y., Li, R., Xiong, T., Wang, J., & Zou, P. (2019). Mapping spatial transcriptome with light-activated p7 KB (989 words) - 17:49, 21 October 2021
- We first ran a control experiment to examine the effect of addition of the beads themsel *<p align="justify"> [2] Sandt, C. H., Y. D. Wang, R. A. Wilson, and C. W. Hill. 1997. Escherichia coli strains with nonimmun28 KB (4,120 words) - 23:56, 13 October 2022
- ...control group, which was entirely in the dark state, the expression of GFP ran up to a high degree of 50,000. <br/> 2. Wang, X., Chen, X. & Yang, Y.(2012) Spatiotemporal control of gene expression7 KB (967 words) - 07:56, 18 October 2012
- ...ost characterization data on hbiF. When swapping out the fimA promoter, we ran in to two problems. The first was that part of the fimA promoter overlapped 5. Wang Q, Calvo JM. Lrp, a major regulatory protein in Escherichia coli, bends DNA4 KB (591 words) - 15:35, 29 September 2013
- ...ost characterization data on hbiF. When swapping out the fimA promoter, we ran in to two problems. The first was that part of the fimA promoter overlapped 5. Wang Q, Calvo JM. Lrp, a major regulatory protein in Escherichia coli, bends DNA4 KB (591 words) - 15:37, 29 September 2013
- ...ost characterization data on hbiF. When swapping out the fimA promoter, we ran in to two problems. The first was that part of the fimA promoter overlapped 5. Wang Q, Calvo JM. Lrp, a major regulatory protein in Escherichia coli, bends DNA4 KB (590 words) - 15:38, 29 September 2013
- ...ost characterization data on hbiF. When swapping out the fimA promoter, we ran in to two problems. The first was that part of the fimA promoter overlapped 5. Wang Q, Calvo JM. Lrp, a major regulatory protein in Escherichia coli, bends DNA4 KB (590 words) - 16:17, 29 September 2013
- ...ost characterization data on hbiF. When swapping out the fimA promoter, we ran in to two problems. The first was that part of the fimA promoter overlapped 5. Wang Q, Calvo JM. Lrp, a major regulatory protein in Escherichia coli, bends DNA4 KB (590 words) - 16:17, 29 September 2013
- <li>Cong L, Ran F A, Cox D, et al. Multiplex genome engineering using CRISPR/Cas systems[J] <li>Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., &43 KB (6,626 words) - 15:52, 16 July 2020
- Majumdar AS, Hughes DE, Lichtsteiner SP, Wang Z, Lebkowski JS, Vasserot AP. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,7 KB (991 words) - 13:50, 17 September 2015
- ...ith all four of our peptides, along with a randomized hybrid of 4B4/4D, we ran into difficulties in gel extracting all but 4D and P601G at a high enough c Fujishima, K., Venter, C., Wang, K., Ferreira, R., & Rothschild, L. (2015). An overhang-based DNA block shu3 KB (421 words) - 15:12, 27 October 2016
- ...ith all four of our peptides, along with a randomized hybrid of 4B4/4D, we ran into difficulties in gel extracting all but 4D and P601G at a high enough c Fujishima, K., Venter, C., Wang, K., Ferreira, R., & Rothschild, L. (2015). An overhang-based DNA block shu3 KB (429 words) - 15:17, 27 October 2016
- ...rotein by 6X His-Tag behind the peptide using Nickel resin column. Then we ran the SDS-PAGE to verify the purification and analyze the concentration of Hv 1. Wang, X.H.; Connor, M.; Wilson, D.C.; Wilson, H.I.; Nicholson, G.M.; Smith, R.;9 KB (1,474 words) - 13:43, 30 October 2016
- ...rotein by 6X His-Tag behind the peptide using Nickel resin column. Then we ran the SDS-PAGE to verify the purification and analyze the concentration of P< 4. Wang, X.H.; Connor, M.; Wilson, D.C.; Wilson, H.I.; Nicholson, G.M.; Smith, R.;10 KB (1,618 words) - 23:17, 30 October 2016
- In order to obtain more information on the localization of eCPX-nSA-cNano, we ran another SDS-PAGE gel on both the cell lysates and the resulting cellular de ....; Sell, B. R.; Allen, J. R.; Day, R. N.; Israelsson, M.; Davidson, M. W.; Wang, J. 2013. A bright monomeric green fluorescent protein derived from <i>Bran26 KB (3,937 words) - 21:45, 10 December 2016
- ...Chemie ''123'', 1116-1118.</ref> and triacetic acid lactone<ref>Frei, C., Wang, Z., Qian, S., Deutsch, S., Sutter, M., and Cirino, P. (2016). Analysis of ...raC_BBPre_F'' and ''araC_BBSuf_R'' were added and a final round of PCR was ran with the following programme: 98°C for 30s, then 30 cycles of 98°C11 KB (1,677 words) - 23:27, 31 October 2017
- ...Chemie ''123'', 1116-1118.</ref> and triacetic acid lactone<ref>Frei, C., Wang, Z., Qian, S., Deutsch, S., Sutter, M., and Cirino, P. (2016). Analysis of ...raC_BBPre_F'' and ''araC_BBSuf_R'' were added and a final round of PCR was ran with the following programme: 98°C for 30s, then 30 cycles of 98°C11 KB (1,671 words) - 23:30, 31 October 2017
- ...Chemie ''123'', 1116-1118.</ref> and triacetic acid lactone<ref>Frei, C., Wang, Z., Qian, S., Deutsch, S., Sutter, M., and Cirino, P. (2016). Analysis of ...raC_BBPre_F'' and ''araC_BBSuf_R'' were added and a final round of PCR was ran with the following programme: 98°C for 30s, then 30 cycles of 98°C11 KB (1,658 words) - 23:30, 31 October 2017
- ...Chemie ''123'', 1116-1118.</ref> and triacetic acid lactone<ref>Frei, C., Wang, Z., Qian, S., Deutsch, S., Sutter, M., and Cirino, P. (2016). Analysis of ...raC_BBPre_F'' and ''araC_BBSuf_R'' were added and a final round of PCR was ran with the following programme: 98°C for 30s, then 30 cycles of 98°C11 KB (1,656 words) - 23:08, 1 November 2017