Part:BBa_K404007

pCMV_[AAV2]-VP123

ViralBricks for Loop replacement

ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops around the positions 453 and 587. Functional peptides are integrated in the loop tip which will be present on the surface of viral vectros that have been modified with the corresponding ViralBrick.

Different ViralBrick versions:

• 453 integration site
• 587 integration site
• 587 integration site with HSPG knock-out

Different functional motifs:

• Biotinylation Acceptor Peptide (BAP)
• His-Affinity tag (His)
• RGD-Motif for Integrin targeting
• Z34C a antibody binding motif from proein A

All these ViralBricks can be used to integrate functional motifs into the viral capsid of the composite part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2396
  Illegal XhoI site found at 698
  Illegal XhoI site found at 884
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 665
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2922
  Illegal SapI site found at 1833