Project

Part:BBa_K404090

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 22:48, 26 October 2010 by Jessica (Talk | contribs)

[AAV2]-Rep40ex

Freiburg10 ReplicationBricks 1.png

Rep40

rep 40
Freiburg10 ReplicationBricks 1.png
BioBrick Nr. BBa_K404090
RFC standard RFC 10
Requirement pSB1C3
Source pAAV_MCS
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

Rep40 in some minutes
• 40 kDa
• ATPase and helicase activity
• Involved in genome encapsidation
 The smallest Rep protein (Rep40) possesses helicase and ATPase activity as well, but does not have strict requirements for DNA duplexes containing a 3´single-stranded end. Rep40 helicase activity requires bivalent ions such as Mg2+ or Mn2+ and is most active using ATP as substrate. Lacking the zinc finger domain, present in Rep52, Rep40 requires dimerization for functional helicase activity (Collaco, Kalman-Maltese, Smith, Dignam, & Trempe, 2003). Rep40/52 proteins are required for translocation of the single-stranded, viral genomes into the preformed capsids proceeding with the 3´end of the DNA (King, Dubielzig, Grimm, & Kleinschmidt, 2001).






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 53
    Illegal XhoI site found at 920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/miscellaneous
Parameters
None