Translational_Unit

Part:BBa_K302034

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 00:04, 26 October 2010 by Registry (Talk | contribs)

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mazEF toxin-antitoxin cluster

Encodes a stable non-specific ribonuclease toxin and its inhibitory antitoxin in Bacillus Subtilis. These genes are use in Bacillus Subtilis to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None