Tag

Part:BBa_K416000:Design

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-05-28)
Revision as of 18:35, 16 June 2010 by RussellDurrett (Talk | contribs) (→‎Design Notes)

Aga2 CDS Responsible for Yeast Surface Display


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have removed the STOP codon of this part to allow for N- and C-terminal Aga2 fusions, which have both been proven to occur efficiently (Refer to Wang, z. et. al. : Protein Engineering, Design & Selection, Vol. 18 No. 7 pp. 337-343, 2005.

Source

This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.

References