DNA

Part:BBa_K416002:Design

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-06-01)
Revision as of 16:05, 16 June 2010 by Hsk299 (Talk | contribs) (→‎Design Notes)

36 Base Pair LoxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a LoxP site, target of Cre Recombinase, normally 34-bp but here 2 bp were added so that the site can be translated in frame with a fusion protein.

Source

Amplified and biobricked from E. coli.

References