Reporter

Part:BBa_K247004:Experience

Designed by: Kaoru Yamamoto,Yoshimi Iyama   Group: iGEM09_Chiba   (2009-10-19)
Revision as of 03:52, 22 October 2009 by Hiroki (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K247004

User Reviews

UNIQ79a6eccd90b0e5d8-partinfo-00000000-QINU UNIQ79a6eccd90b0e5d8-partinfo-00000001-QINU

This part properly acted as RFP reporter.

Excitation peak: 584 nm, Emission peak: 607 nm


The Chiba iGEM team tested this part in several conditions. Experiment Condition

  • AHL concentration of medium : 100 nM
  • Time when we observed mRFP fluorescence : 24 h (since we permeated AHL to this RFP)
  • medium : 1(left) or 5%(right) agar and 10 mL of LB-Amp, Cm medium
Fig. 1　RFP Fluorescent. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.