Primer

Part:BBa_K5492010

Designed by: Norbert Nyeste   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-17)
Revision as of 14:48, 1 October 2024 by Noriii (Talk | contribs)


Twist adapter forward primer

Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.

Experiments

PCR - optimised DAO

We have found the following PCR protocol the most optimal for the part: dao-pcr-plan.png


We added the following substrates in order:

dao-pcr-substr.png


We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order:


opt-dao-pcr.png

1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None