Primer

Part:BBa_K5492010

Designed by: Norbert Nyeste   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-17)
Revision as of 14:27, 1 October 2024 by Noriii (Talk | contribs)


Twist adapter forward primer

Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.

Experiments

PCR

We have found the following PCR protocol the most optimal for the part: dao-pcr-plan.png

Optimised dao:

We added the following substrates in order:

dao-pcr-substr.png



We added the 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order: 1. D31O 2. D32O 3. D33O 4. D34O 5. Ladder


opt-dao-pcr.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None