Part:BBa_I13453:Experience

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

••••• kahaynes

pBad I13453 confers expression of tetracycline resistance when placed upstream of hixC-RBS-Tet... (BBa_J44010). The presence of hixC (26 bp) between pBad and RBS-Tet does not disrupt transcription. In the insulated vector pSB1A7 we have observed that tetracycline resistance depends upon the presence and proper orientation of pBad relative to the coding region (Davidson and Missouri Western 2006). pBad-hixC-RBS-Tet is tetracycline resistant, whereas RBS-Tet and pBad-hixC-Tetrev-RBSrev are not. We have observed Tet expression in the absence of L-arabinose (the affects of L-arabinose were not tested in this system). In summary, pBad I13453 (which includes the Cap and AraC protein binding domains and polymerase binding site; Lee, PNAS, 1981) appears to be a functional constitutive promoter that does not depend upon L-arabinose for expression.

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