Composite

Part:BBa_K5097011

Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora   Group: iGEM24_Oneonta   (2024-09-30)
Revision as of 14:55, 30 September 2024 by Elberlopezh (Talk | contribs)


Acid-sensor, Base-producer pASR-PUT1

Usage and Biology

This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2930
    Illegal BamHI site found at 1541
    Illegal BamHI site found at 2843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1319
    Illegal AgeI site found at 2865
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None