Part:BBa_K5097009

SpeA (arginine decarboxylase)

Usage and Biology

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.

Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)

References

Shaibe, E et al. “Metabolic pathway for the utilization of L-arginine, L-ornithine, agmatine, and putrescine as nitrogen sources in Escherichia coli K-12.” Journal of bacteriology vol. 163,3 (1985): 933-7. doi:10.1128/jb.163.3.933-937.1985

Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.

Charlier, Daniel & Bervoets, Indra. (2019). Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli. Amino Acids. 51. 10.1007/s00726-019-02757-8.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1389
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1167
  
• 1000
  COMPATIBLE WITH RFC[1000]

This gene sequence has been altered to make the SpeA gene RCF10 compatible. To do this, we removed an internal PstI restriction sites, as follows G291A, T1170G, G1434A. These changes will not impact the amino acid sequence.