Composite

Part:BBa_K5416030

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-29)
Revision as of 11:22, 29 September 2024 by Registry (Talk | contribs)

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PhaP PhaC-HRT2trunc

This composite part encodes a polyhydroxyalkanoate(PHA) phasin protein PhaP (BBa_K208001) and a fusion protein of PHA synthase PhaC from C. necators fused with rubber synthase HRT2trunc (BBa_K5416000). The CDS were placed in between a t7-lacO promoter (BBa_K1624002) and a t7 terminator (BBa_K731721) for IPTG inducible expression. This part is designed to enable the formation of rubber particles as an artificial organelle through a mechanism inspired by the formation of PHA granules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None