Part:BBa_K5366050

Gene Cluster

During the experiment, it was observed that the biosensor responded to the target substance by transporting tagatose from the environment into the cells. Escherichia coli Nissle 1917 (EcN) has been demonstrated to utilize tagatose as a carbon source due to the presence of a series of gene clusters related to tagatose metabolism, including tagatose transporters. These gene clusters play a crucial role, as they not only encode specific transporters that effectively recognize and transport tagatose, but also include various enzymatic genes that enable the rapid breakdown and utilization of tagatose once it is inside the cells. This process significantly enhances the absorption efficiency of tagatose, allowing E.coli Nissle 1917 to grow and replicate swiftly in a tagatose-rich environment.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3646
  Illegal EcoRI site found at 4094
  Illegal PstI site found at 479
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3646
  Illegal EcoRI site found at 4094
  Illegal PstI site found at 479
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3646
  Illegal EcoRI site found at 4094
  Illegal BamHI site found at 290
  Illegal BamHI site found at 3733
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3646
  Illegal EcoRI site found at 4094
  Illegal PstI site found at 479
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3646
  Illegal EcoRI site found at 4094
  Illegal PstI site found at 479
  Illegal AgeI site found at 1730
  Illegal AgeI site found at 4617
  Illegal AgeI site found at 5112
  
• 1000
  COMPATIBLE WITH RFC[1000]