Composite

Part:BBa_K5461000

Designed by: Jiang Nan   Group: iGEM24_LCG-China   (2024-09-24)
Revision as of 08:20, 24 September 2024 by Registry (Talk | contribs)

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GST-csgA-spytag

CsgA is a biofilm protein that does not assemble within E. coli but can self-assemble into fibrous structures when expressed in vitro. This part is designed to express a protein with a GST purification tag and a SpyTag oligopeptide, under the control of the T7 promoter. We have expressed the fusion protein csgA-SpyTag with a GST tag within E. coli and successfully purified it using column chromatography. In our system, the csgA-SpyTag fusion protein serves as a linker between cellulose and the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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