Part:BBa_K5492202
Wild type rDAO - pETDUET-1
Wild type coding sequence of rat Diamine Oxidase appropiated for insertion into pETDUET-1.
Usage and Biology
Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 40
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 40
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 40
Illegal BglII site found at 1050
Illegal BglII site found at 2303
Illegal BamHI site found at 1978
Illegal BamHI site found at 2232
Illegal XhoI site found at 848 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 40
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 40
- 1000COMPATIBLE WITH RFC[1000]
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