Part:BBa_K5492402

Wild type HNMT - pETDUET-1

Wild type coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into pT7CFE1.

Usage and Biology

Histamine-N-Methlytransferase (HNMT) takes part in the inactivation of histamine. It transfers a methyl group to histamine, and the resulting N-methyl histamine isn’t able to bind to histamine receptors, thus reducing the biological effect of the compound. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 129
  Illegal EcoRI site found at 235
  Illegal XbaI site found at 47
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 129
  Illegal EcoRI site found at 235
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 129
  Illegal EcoRI site found at 235
  Illegal BglII site found at 1015
  Illegal BamHI site found at 123
  Illegal XhoI site found at 457
  Illegal XhoI site found at 1064
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 129
  Illegal EcoRI site found at 235
  Illegal XbaI site found at 47
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 129
  Illegal EcoRI site found at 235
  Illegal XbaI site found at 47
  Illegal NgoMIV site found at 1034
  
• 1000
  COMPATIBLE WITH RFC[1000]