Part:BBa_K5396000
BaCBM2_RFP_3xMad10
This CBM2 protein is fused with the red fluorescent protein (RFP), which exhibits an excitation maximum at 558 nm and an emission maximum at 583 nm. This fusion enhances the visualization of CBM2. The protein also has three MAD10 peptides [ ], which serve as a magnetic tag that facilitates the purification of the protein through magnetic separation techniques.
This part was used as template to construct BBa_K5396003.
Usage and Biology
This CBM2, or Carbohydrate-Binding Module 2, is a protein sourced from Bacillus anthracis. It belongs to a broader family of carbohydrate-binding modules that are crucial for the degradation of polysaccharides. These modules are important to break down complex carbohydrates, enabling microorganisms to convert them into usable energy sources.
Recent study [ ] has shown that CBM2 has the ability to bind to certain types of plastics, especially those derived exhibiting similar structural features of polysaccharides. This binding ability is largely due to the protein's carbohydrate-binding properties, which facilitate interactions with specific functional groups found on plastic surfaces.
Figure 1. AlphaFold 3 3D simulation of BaCBM2 with miRFP and three Mad10 tags.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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