Plasmid

Part:BBa_K5492102

Designed by: Norbert Nyeste   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-16)
Revision as of 11:24, 16 September 2024 by Registry (Talk | contribs)

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pT7CFE1-CHis

pETDUET-1 plasmid used for expression of Histamine-N-Methyltransferase and Diamine Oxidase in e.coli chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 557
    Illegal SpeI site found at 680
    Illegal PstI site found at 578
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 557
    Illegal SpeI site found at 680
    Illegal PstI site found at 578
    Illegal NotI site found at 596
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 557
    Illegal BglII site found at 631
    Illegal BamHI site found at 551
    Illegal XhoI site found at 605
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 557
    Illegal SpeI site found at 680
    Illegal PstI site found at 578
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 557
    Illegal SpeI site found at 680
    Illegal PstI site found at 578
    Illegal NgoMIV site found at 3330
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1846


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