Part:BBa_K5036021
UAS Trans CMV enhancer
Part Description
it is a a Powerful Tool for Gene Expression which is derived from the cytomegalovirus (CMV). it can significantly boost gene expression in various cell types. it works by binding to specific transcription factors, which then recruit other proteins to the promoter region of a gene. This complex interaction helps to initiate and sustain gene transcription.
Usage
This part is fused with GAL4, dcas9(c), VP64. it acts as a powerful transcription activator where it induce transcription and increased gene expression so after receptor activation, it will be directed with dCas9,VP64 and GAL4 to increases expression of YAP.
Literature Characterization
Three AAV-2 vectors were created by inserting different promoters (CMV enhancer, PDGF, or a hybrid of the two) into a plasmid containing a firefly luciferase gene. These plasmids were then combined with two other plasmids (pAAV-RC and pHelper) and introduced into HEK293 cells which resulted in the production of three AAV-2 vectors, named AAV-CMV enhancer-luc, AAV-PDGF-luc, and AAV-CMV enhancer/PDGF-luc. The researchers tested how well the three AAV-2 vectors infected different types of cells, including primary rat brain cells (neurons and glial cells), and mouse and human cell lines (C17.2 and NT2-N) that had been grown to become neurons. They used a high dose (MOI of 1000) to ensure maximum infection.
AAV-2 vectors containing a hybrid CMV enhancer/PDGF promoter significantly increased luciferase gene expression in primary cortex neurons compared to vectors with the CMV enhancer/P promoter or the PDGF promoter alone respectively, at both 4 and 7 days post-transduction (Figures 1A and 1B).. This effect was seen in both mouse and human neuronal cells. However, in primary glial cells, the CMV enhancer/P promoter was more effective than the hybrid promoter(Figure 1C). These results suggest that the hybrid promoter retains its neuronal specificity.
ٌReference
Wang, C. Y., Guo, H. Y., Lim, T. M., Ng, Y. K., Neo, H. P., Hwang, P. Y. K., ... & Wang, S. (2005). Improved neuronal transgene expression from an AAV‐2 vector with a hybrid CMV enhancer/PDGF‐β promoter. The Journal of Gene Medicine: A cross‐disciplinary journal for research on the science of gene transfer and its clinical applications, 7(7), 945-955.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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