Signalling

Part:BBa_K5036002

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-09)
Revision as of 17:43, 10 September 2024 by Em100 (Talk | contribs) (→‎Usage)


NLS

Part Description

It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) to keep it in the nucleus to avoid dCas9 self assembly.

Usage

This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None