Coding

Part:BBa_K5043004

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)
Revision as of 17:56, 17 August 2024 by Registry (Talk | contribs)

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phdF from M. vanbaalenii PYR-1

Encodes ring cleavage dioxygenase presumably catalysing reaction of 4,5-dihydroxypyrene to Phenanthrene-4,5-Dicarboxylate. This is the third step in pyrene biodegradation. This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrader.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
    Illegal AgeI site found at 470
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None