Translational_Unit

Part:BBa_K4948033

Designed by: Chris Song   Group: iGEM23_Seoul-Korea   (2023-10-12)
Revision as of 15:28, 12 October 2023 by Envien mc (Talk | contribs)


PJEx-D-RBS-vsfGFP-0_lva

One of the plaamids comprising our biosensor, MoNitro, continuously produces GFP under normal conditions under the control of the PJEx-D promoter. However, when exposed to high concentrations of nitrate, the expression of the Mf_lon protease and EilR repressor, which are regulated by the nitrate inducible promoter, is overwhelmed, resulting in the degradation of lva-tagged GFP, and the transcription of the PJEx-D promoter is inhibited by EilR, resulting in low GFP levels.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 199
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 199
    Illegal NgoMIV site found at 451
    Illegal AgeI site found at 557
    Illegal AgeI site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None