Composite
pAND

Part:BBa_K4634013

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-06)
Revision as of 18:04, 6 October 2023 by NgSweeHong (Talk | contribs)


Single Plasmid AND GATE

AND GATE is a composite part to process two different signal. For this particular AND GATE, temperature and arabinose signal are detected. After undergo heat-shock treatment, T7ptag is transcribed. But as the T7ptag mRNA consist amber stop codons, it cannot be translated to fully functional protein. Meanwhile, if arabinose is added to the system, SupD tRNA is transcribed. In the presence of SupD, T7ptag mRNA can be translated to a functional T7 RNA polymerase. Hence the mCherry gene controlled by T7 promoter is expressed and fluorescent signal is detectable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2502
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1245
    Illegal AgeI site found at 2261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1248
    Illegal SapI site found at 961


References

[1] Anderson JC, Voigt CA, Arkin AP. Environmental signal integration by a modular AND gate. Mol Syst Biol. 2007;3:133. doi: 10.1038/msb4100173. Epub 2007 Aug 14. PMID: 17700541; PMCID: PMC1964800.

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