Regulatory

Part:BBa_K190023

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-08-25)
Revision as of 18:39, 18 October 2009 by PaulSchavemaker (Talk | contribs)

Arsenic Promoter (ArsR regulated) with own RBS

Promoter sequence containing the recognition site for ArsR transcriptional regulator protein. Downstream from the promoter is the RBS that is attached to this promoter region in the E. coli genome. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, thereby activating transcription (1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


1) C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.

[edit]
Categories
//function/regulation/transcriptional
//promoter
//regulation/negative
Parameters
control
negative_regulators