Composite

Part:BBa_K4674013

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)
Revision as of 15:06, 8 October 2023 by Registry (Talk | contribs)

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The PBSII-Zif268 expression cassette

This expressing cassette is regulated by lac operator. The N-terminal of PBSII-Zif268 protein is fused with a 6xHis-tag and a thrombin cleavage site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 424
    Illegal BamHI site found at 711
    Illegal XhoI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None