Status: 500 Content-type: text/html

Software error:

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Revision as of 03:51, 12 October 2023 by Kaga (Talk | contribs)


Status: 500 Content-type: text/html

Software error:

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Usage and Biology

In order to observe the decomposition of PET by engineered bacteria, our team designed this plasmid. The plasmid mainly consists of RFP, lox66&lox71, PHpaII, Xylsmut, tetO and sfGFP genes. It is mainly used to detect and report the presence of PA and TPA, and visually monitor the expression through the GFP.When the TPA is detected by the Xylsmut, the downstream genes will be activated and transcribed——like the strain will express green fluorescence.

Fig 1. The expression level of the monellin.

What’s more, considering that in future product applications, the strain may not necessarily be used to degrade PET immediately after delivery, we designed the flipping system to cope with different use situations. During the strain is put into use to decompose the PET, we can regulate the flipping sequence to detect the presence of TPA and activate the expression of GFP. In other situations, flipping expresses the RFP and plays an alternative role. For example, when applied in a factory, in order to reduce adverse effects during fermentation production, the engineered bacteria express antibacterial products before turning over, and then turn over to express downstream degradation modules until they are put into use. Or in the field, the strain produces substances that increase fertility into the soil before the sequence is flipped. In turn, the engineered bacteria could have more purposes.

Fig 2. The expression level of the monellin.

Sequence and Features Status: 500 Content-type: text/html

Software error:

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Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
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BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.

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