Part:BBa_K4994002
Arabinose promoterr+SRRz cassette
p-Cresol is an organic compound with a chemical formula of C7H8O. Its molecular structure includes a hydroxyl group (OH) and a methyl group (CH3). Despite its various industrial and laboratory applications, it is considered a hazardous substance that can have adverse effects on human health. Prolonged exposure to and inhalation of p-Cresol can lead to various adverse reactions, including skin irritation and respiratory irritation. In more severe cases, it may even have detrimental effects on vital organs such as the central nervous system and the liver. In industrial production and laboratory settings, p-Cresol is typically used as a solvent, raw material, and disinfectant, among other purposes. Therefore, we have decided to develop a biocensor for monitoring p-Cresol based on Escherichia coli (E. coli). The colorimetric system involves the catalytic conversion of p-Cresol into 4-methylquinone by tyrosinase, which is further oxidized to 4-methyl-o-quinone. In the presence of MBTH (3-methyl-2-phenyl-2H-indazol-6-amine), this substance rapidly transforms into a pink complex, providing a visual indication of the presence or changes in p-Cresol concentration, offering an intuitive detection method. In addtion，the SRRz gene cluster is composed of a linked set of genes, including the S gene, the R gene (encoding a soluble transglycosylase enzyme that degrades peptidoglycan in the cell wall), and the RZ gene (encoding an endopeptidase enzyme that cleaves between oligosaccharides in peptidoglycan and crosslinks between peptidoglycan and the outer membrane of the cell). The product of the S gene functions to alter the permeability of the cell membrane, forming a porous structure on the membrane, allowing the enzymes produced by the R and RZ genes to pass through the membrane and reach the cell wall. As a result, the cell wall is acted upon, leading to its rupture and the release of cellular contents. Therefore, the SRRz gene cluster facilitates cell wall disruption.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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