Coding

Part:BBa_K4837001:Design

Designed by: Tse Cheong Tat   Group: iGEM23_HK-SKHLPSS   (2023-10-11)
Revision as of 00:16, 12 October 2023 by Lpctt (Talk | contribs)


Manganese peroxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
    Illegal NgoMIV site found at 1153
    Illegal AgeI site found at 651
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865
    Illegal SapI.rc site found at 523


Design Notes

pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.

Other biobricks such as BBa_K4837000 are required to perform its function.


Source

It is designed based on part of white-rot fungus (Phanerochaete chrysosporium).

References