Part:BBa_K4779003

CMPS Pro:A yeast copper-induced reporter pathway with Positive feedback loop with prm1pro promoter

It is a copper ion reaction reporting pathway in Saccharomyces cerevisiae. We utilized the pprm1 Pro for optimizing GFP expression, and employed the prm1 promoter to express Ste5ΔN-CTM for positive feedback regulation in the yeast copper-induced reporting pathway.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3138
  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal SpeI site found at 4393
  Illegal SpeI site found at 4939
  Illegal PstI site found at 49
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3138
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal SpeI site found at 4393
  Illegal SpeI site found at 4939
  Illegal PstI site found at 49
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3138
  Illegal BglII site found at 55
  Illegal BglII site found at 3586
  Illegal BglII site found at 4894
  Illegal BamHI site found at 510
  Illegal XhoI site found at 8
  Illegal XhoI site found at 4831
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3138
  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal SpeI site found at 4393
  Illegal SpeI site found at 4939
  Illegal PstI site found at 49
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3138
  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal SpeI site found at 4393
  Illegal SpeI site found at 4939
  Illegal PstI site found at 49
  Illegal NgoMIV site found at 36
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2694
  Illegal SapI site found at 529